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Environment and Natural Resources Institute
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Ion Chromatography
Major anion analyses are performed using IonPac As15 (4mm) column, and self regenerating suppressor ASRS Ultra (4mm); sample loop is 25 μL.
Anion analyzed:
| retention time (min) | | Fluoride | 3.8 | | Chloride | 4.5
| | Nitrite | 6.2
| | Sulfate | 7.2 | | Bromide | 12.6< | | Nirate | 15 | | Phosphate | 20 |
Samples should be filtered through 0.45μm filters immediately after sampling and stored at 4 ºC. The shelf time of some anions is only 48 hours. The analysis is performed using calibration standard at 4 levels adjusted to concentration of the samples. Quality control and blank control is performed every 10th sample. For a full SOP click .
Perchlorate is separated using IonPac AS16 (2 mm) column and self regenerating suppressor ASRS Ultra II (2mm) with external water mode. KOH
| Eluent | Isocratic
| 65 mM
| Flow rate
| <0.25 mL/min
| Total time
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14 minutes
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Anion analyzed:
| Perchlorate
| retention time
| 12.5 min
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Detection is performed with conductivity detector in water matrixes. For complex matrixes (e.g. tissue) the IC is connected to a mass spectrometer (Finnigan AQA) with an electrospray ionization source. The settings of mass spectrometer are:
Negative mode
| -ve
| AQA mix
| 5 V
| probe temperature
| 300 C
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For further details on our pechlorate analyses see Dodds, et al. (2004), Anal. Bioanal. Chem. 379:881-887. © 2008 ASET Laboratory
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