Major anion analyses are performed using IonPac As15 (4mm) column, and self regenerating suppressor ASRS Ultra (4mm); sample loop is 25 μL.
Anion analyzed:
| Fluoride
| retention time (min) | 3.8
|
| | Chloride | | 4.5 |
| | Nitrite | | 6.2 |
| | Sulfate | | 7.2 |
| | Bromide Nirate | | 12.6 15
|
| | Phosphate
| | 20
|
Samples should be filtered through 0.45μm filters immediately after sampling and stored at 4 ºC. The shelf time of some anions is only 48 hours. The analysis is performed using calibration standard at 4 levels adjusted to concentration of the samples. Quality control and blank control is performed every 10th sample. For a full SOP click . Perchlorate is separated using IonPac AS16 (2 mm) column and self regenerating suppressor ASRS Ultra II (2mm) with external water mode.
| Eluent | KOH
|
Isocratic
| 65 mM
|
Flow rate
| 0.25 mL/min
|
Total time
| 14 minutes
|
Anion analyzed:
| Perchlorate
| retention time
| 12.5 min
|
Detection is performed with conductivity detector in water matrixes. For complex matrixes (e.g. tissue) the IC is connected to a mass spectrometer (Finnigan AQA) with an electrospray ionization source. The settings of mass spectrometer are:
Negative mode
| -ve
|
AQA mix
| 5 V
|
probe temperature
| 300 C
|
For further details on our pechlorate analyses see Dodds, et al. (2004), Anal. Bioanal. Chem. 379:881-887.
© 2008 ASET Laboratory
