Major anion analyses are performed using IonPac As15 (4mm) column, and self regenerating suppressor ASRS Ultra (4mm); sample loop is 25 μL.Perchlorate is separated using IonPac AS16 (2 mm) column and self regenerating suppressor ASRS Ultra II (2mm) with external water mode. KOH
Samples should be filtered through 0.45μm filters immediately after sampling and stored at 4 ºC. The shelf time of some anions is only 48 hours. The analysis is performed using calibration standard at 4 levels adjusted to concentration of the samples. Quality control and blank control is performed every 10th sample. For a full SOP click here.
Detection is performed with conductivity detector in water matrixes. For complex matrixes (e.g. tissue) the IC is connected to a mass spectrometer (Finnigan AQA) with an electrospray ionization source. The settings of mass spectrometer are:
For further details on our pechlorate analyses see Dodds, et al. (2004), Anal. Bioanal. Chem. 379:881-887.
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